GENEWIZ features the most cutting-edge next generation sequencing platforms including the new PacBio Sequel, the powerful Illumina HiSeq X Ten, Illumina HiSeq, Illumina MiSeq, and the Ion PGM™. We are a Certified Service Provider (CSPro) for both the Illumina and Ion Torrent sequencing technologies, which guarantees the customer the best data quality.
Illumina Platforms & Specifications
Illumina sequencing platforms utilize sequencing by synthesis (SBS) methodology to perform massively parallel sequencing by detecting single bases as they are incorporated into growing DNA strands. As each dNTP is added to the growing strand, a fluorescently labeled reversible terminator is imaged and then cleaved to allow incorporation of the next base. As all 4 reversible terminator-bound dNTPs are present during each cycle, incorporation bias is minimized, which results in true base-by-base sequencing providing accurate data for a broad range of applications. The fidelity of the method virtually eliminates errors associated with strings of repeated nucleotides (homopolymers).
PacBio Sequel System – NEW!
The PacBio Sequel System platform is based on PacBio’s proven Single Molecule, Real Time (SMRT) sequencing technology.This SMRT sequencing on the PacBio Sequel combines long read technology and rapid sequencing with 7 times higher throughput compared to previous systems for the highest-quality data. The PacBio Sequel is ideal for whole genome de novo assembly projects that require higher-quality data, microbial genome assembly, identifying DNA modications without bisulfite treatment, and identifying large structural variants. The system delivers up to 99.999% accuracy. Request a Quote
The Illumina HiSeq X Ten System is the most powerful sequencing platform created to date for whole genome sequencing. The system consists of a set of 10 HiSeq X ultra-high-throughput instruments that can deliver over 18,000 human genomes per year. The HiSeq X Ten makes human whole genome sequencing more affordable and accessible than ever before by providing ultra-high throughput at an unprecedented price point per genome. In addition, the system also provides great cost and turnaround time advantages over other platforms for population- and production-scale sequencing of non-human species.
The Illumina HiSeq system is one of the most powerful sequencing machines on the market and is used by most major genome centers and leading institutions around the world. This machine offers ultra-high-throughput sequencing for a myriad of project types. Sequencing is conducted either in the High Output Mode (8 lane flowcell) or Rapid Run Mode (2 lane flowcell). GENEWIZ can accept most projects involving custom indexing and sequencing primers, as well as projects that have different read1 and read2 lengths using the Rapid Run Mode on the HiSeq.
The Illumina MiSeq is a smaller benchtop version of the Illumina HiSeq for smaller throughputs. This machine offers ultra-fast sequencing with many flexible options. Read lengths are up to 300bp paired-end, which exceeds that of the HiSeq. GENEWIZ can accept most projects involving custom indexing and sequencing primers, as well as projects that have different read1 and read2 read lengths on the MiSeq.
The 10X GENOMICS Chromium™ Controller enables a diverse set of sequencing applications like whole-genome phasing and structural variant analysis, remapping of difficult regions in the genome, de novo genome assembly, and dynamic gene expression at the single cell level.