CRISPR VALIDATION

Recent advancements in genome editing techniques, namely CRISPR/Cas9, have greatly facilitated cell line engineering, gene targeting, and the development of transgenic models. However, identification of correctly targeted cells remains a significant bottleneck for large-scale projects.​

To remove this hurdle, GENEWIZ developed genoTYPER-NEXT™, a proprietary NGS-based assay ideal for ultra-sensitive, high-throughput, sample-to-answer genotyping projects. This tool is more effective and efficient than T7E1, TIDE, and IDAA assays for screening targeted cell lines.​

We also offer targeted, whole genome, and amplicon sequencing NGS solutions to aid on- and off-target analysis of genome editing.​

 

  

genoTYPER-NEXT: HIGH-THROUGHPUT ASSAY FOR CRISPR VALIDATION

1. SUBMIT SAMPLES

Submit CRISPR-edited cell lines in 96-well plates​

2. AMPLIFY ON/OFF-TARGET SITE(S)

Cells lysis and PCR with barcoded primers​

3. SEQUENCE​

Samples pooled and sequenced on Illumina® platform​

4. ANALYZE DATA INTERACTIVELY​

Visualize results in genoTYPER-NEXT browser​

View our other NGS services for CRISPR Validation

Features & Benefits


Complete sample-to-answer solution starting from cells to genotype (explore our interactive report; log in required)

Automated, high-throughput workflow – Up to 10,000 samples per run​

Hassle-free - Avoid gDNA extraction, pre-screening prior to sequencing, and TA cloning​

Highly sensitive – Detection of <1% allele frequency, full INDEL resolution, and frameshift analysis
More reasons to choose GENEWIZ

Case Study | Rapid, Scalable, and Ultra-Sensitive Validation of Allele-Specific Gene Editing Events with genoTYPER-NEXT™​

Discover the powerful capabilities of genoTYPER-NEXT in this illuminating case study and learn how this high-throughput assay allows rapid and accurate genotyping of CRISPR-edited cell lines.​

Webinar | Rapid and Sensitive Validation of Gene Editing with Novel NGS Assays​​

Learn about current techniques to validate gene edits and how recent advancements in next generation sequencing are improving the sensitivity and scalability of screening genomic manipulations.​

How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1) | Live Chat